Geminate recombination of CO in rabbit, opossum, and adult hemoglobins

The geminate recombination of CO with Hb following dissociation by a 10-ns laser pulse has been studied as a function of pH (9.2 and 7.0 without inositol hexaphosphate and 6.0 with inositol hexaphosphate) and temperature (5-35 degrees C). The hemoglobins studied included adult, Rothschild, rabbit, opossum, and carp. Despite significant differences in their structural and functional properties, the first four of these hemoglobins show similar trends in the yields, rates, and activation energies of the geminate recombination. The nature of the "cage recombination" in hemoglobin is discussed in the light of such findings. Neither a slow diffusion model nor a model based upon a specific non-heme binding site accounts for the observations.     

A possible adenine nucleotide storage form in normal and ischaemic rat heart

Perfusion of Langendorff rat hearts with [¹⁴C]adenosine yields an acid-insoluble, radioactive product whose concentration falls during ischaemia. The properties of the substance show that it is a polyribonucleotide. It is suggested that it may be mitochondrial poly A acting as a storage form of adenine nucleotides.     

Dystocia due to urinary bladder carcinoma in two water buffaloes (bubalus bubalis) - Clinical case report

Two buffaloes with full-term pregnancy suffered from dystocia because the cervix did not dilate in spite of strong labour pains and other parturition signs shown by each animal. The urinary bladder, cervix, vagina and surrounding area were very firm. Dead, emphysemated fetuses were removed by caesarean in each case and anuria was also noticed. One buffalo died and the other was euthanised after surgery because it did not improve. The post-mortem examinations revealed transitional cell carcinoma of urinary bladder infiltrating the cervix, vagina and surrounding area in each case.     

A note on some Eurotatoria from Panjab State,India

Among 35 eurotatorian species presently reported from Panjab State, India, ten species are new records to this region while eight are new records from N. W. India. A new synonym is proposed and biogeographical remarks are made.     

Synthesis and conformational studies of 2-beta-chloro, 1-alpha-fluoro, and 2-beta-fluoro derivatives of 2-deoxy-N-acetyl-neuraminic acid

5-Acetamido-4,7,8,9-tetra-O-acetyl-2,3,5-trideoxy-2-fluoro-D-glycero-alp ha- and -beta-D- galacto -2- nonulosonic acid methyl esters and the beta-chloro analog were synthesized from N-acetylneuraminic acid. Their 1H- and 13C-n.m.r.spectra were completely assigned by using single-frequency decoupling, off-resonance decoupling, and spin-simulation programs. Bond angles estimated from the 1H coupling-constants indicate that all of the compounds adopt the 2C5 (L) conformation with minor conformational differences in the C3 side chain. 5-Acetamido-2,3,5,-tri-deoxy-2-fluoro-D-glycero-alpha- and -beta-D- galacto -2- nonulosonic acid and their methyl esters were also prepared.     

Nitrate reductase,nitrite reductase activities and leaf diffusive resistance in wheat under water deficit

The flag and second leaves of wheat showed physiological and biochemical differences in the normal and water stressed plants. Although the flag leaf had lower enzyme activities in the control plants, in stressed plants it could continue nitrate assimilation better than the second and other leaves. The flag leaf also exhibited a higher tendency to resume normal metabolic activity after the release of stress as is indicated by higher NRA, NiRa, lower r_ad and r_ab.